PCTS Gene-Editing Sequencing:
From single-base edits to large knock-ins, our probe-based targeted sequencing service maps every edit event with high accuracy and cost-efficiency, optimized for large-scale sample analysis.
Introduction
The advent of gene editing technologies, including CRISPR/Cas9, has revolutionized genetic research and therapeutic applications. However, understanding the precise outcomes of these edits—especially in multi-site editing scenarios—remains a costly challenge. We propose a novel "Gene Editing Site Sequencing Service" utilizing a probe capture targeting sequencing (PCTS) approach. This service provides a comprehensive view of edited sequences, capturing variations across hundreds or thousands of samples, thereby enhancing the understanding of gene editing results.
Why Choose Our Gene Editing Site Sequencing Service?
Gene editing technologies like CRISPR/Cas9 have transformed research, but analyzing the outcomes of multi-site edits remains a challenge. Our PCTS service addresses this by offering comprehensive, high-throughput sequencing that captures the full scope of genetic modifications. Whether you're working on crop improvement, therapeutic development, or fundamental genetic research, our service empowers you to make informed decisions with confidence.
Q: How does the PCTS sequencing work?
Our proposed method, PCTS, leverages the following key components:
1. Custom Probe Design: Specific probes are designed to target the regions of interest, ensuring high specificity for the edited sites while accommodating sequence diversity.
2. Hybridization and Capture: The probes hybridize to the target sequences, allowing for efficient capture of the edited regions.
3. Next-Generation Sequencing (NGS): The captured sequences are sequenced using NGS technology, providing a comprehensive view of all edited sites across a large number of samples.
Key Benefits:
- Comprehensive Coverage: PCTS captures all edited sites, providing a full view of the genetic modifications made.
- High Sensitivity: The method is highly sensitive, allowing for the detection of low-frequency variants, and different versions of edited sites.
- Scalability: PCTS is designed to handle hundreds or thousands of samples simultaneously, making it suitable for large-scale projects.
- Cost-Effectiveness: By streamlining the sequencing process, PCTS reduces costs associated with data generation and analysis.
Sorghum Research Success: “The PCTS service from MolBreeding gave us a detailed view of small fragment deletions in our sorghum lines, it detects SNPs, small InDels, large deletions, not just simple presence/absence. Thanks to its efficiency and affordability, we could scale up and analyze more samples at record speed.”
Our Service Workflow
Our PCTS Service workflow is streamlined to deliver fast, accurate results:
1. Sample Preparation: DNA is extracted from the samples, and libraries are prepared for sequencing.
2. Probe Hybridization: Custom probes are introduced to hybridize with the target gene editing sites.
3. Sequence Capture: Hybridized sequences are captured and purified for sequencing.
4. Sequencing: The captured DNA is sequenced using NGS platforms, generating detailed sequence data.
5. Data Analysis: Advanced bioinformatics tools are utilized to analyze the sequence data, identifying edited sites, variations, and off-target effects.
Report Visualization
The detection report displays the haplotype distribution of target regions across various samples, providing a clear interpretation of mutation frequency and sequencing depth in the gene editing areas.
Detection of Various Mutation Types
Large Deletion Results in Target Regions
Applications
Our Gene Editing Site Sequencing Service is optimized for large-scale sample analysis, making it an ideal solution for high-throughput genetic research. Key applications include:
- Agricultural Biotechnology: Evaluate genetic modifications in crops to enhance traits like yield, disease resistance, and environmental adaptability.
- Therapeutics: Evaluating the efficacy and safety of gene editing cell lines in clinical settings.
- Basic Research: Explore gene function and regulation with precise sequence analysis.
Customer Testimonials: "Working with probe capture targeting sequencing (PCTS) services has speed up our gene editing detection process for maize. The customized report provided clear insights into variant detection and transgene presence, making it easier for us to advance our research."
The future of gene editing with PCTS
The PCTS-powered Gene Editing Site Sequencing Service is transforming the landscape of genetic research. By delivering detailed insights into edited sequences across multiple sites and thousands of samples, we’re enabling researchers to achieve more precise and impactful outcomes. From enhancing crop resilience to advancing precision medicine, our service is driving innovation and unlocking new possibilities in gene editing.
Get Started Today
We invite researchers, institutions, and biotech companies to collaborate with us in utilizing this innovative sequencing approach to enhance their gene editing projects. For more information or to schedule a consultation, please contact us.
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