GenoPlex-One™
One-Step Multiplexing PCR and Sequencing
GenoPlex-One is an innovative genotyping solution designed to streamline the multiplexing of thousands of DNA samples while analyzing hundreds of SNPs in a single PCR step. This approach allows for pooling in one reaction for NGS sequencing.
Focusing on efficiency, high-throughput capabilities, and accuracy, GenoPlex-One enables researchers to conduct large-scale genetic testing - all within a single day.
Want to learn more about our GBTS marker panel? Click here
Curious about how other researchers have used GBTS? Check out peer-reviewed papers.
Key Features
- One-Step Multiplexing: Genotype dozens to hundreds of SNPs in a single PCR reaction.
- Flexible Target Design: Cost-effective targeted panels supporting 20–500 markers.
- Simplified, Cost-Efficient Workflow: One PCR stage, one plate—no transfers, lower contamination risk and costs.
- Ultimate Throughput: Process 6,000–10,000 samples per day for large-scale genotyping.
- Integrated Data Analysis: End-to-end SNP analysis powered by MolBreeding.
Following a one-step PCR amplification (~2.5 hours) and a quick pooling/purification step (~1 hour), this process allows for the genotyping of
hundreds of SNPs in a single, efficient workflow.
Performance
In the evaluation of three GenoPlex-One panels—Maize, Pepper, and Tomato—demonstrated consistently high performance, with call rates and uniformity exceeding 94%. This performance was reliably maintained across replicates. All panels achieved over 90% alignment to the reference genome with comparable call rates.
The Performance of three Genoplex-One panels:
Bars are colored from light to dark (left → right) to indicate : Alignment Rate (%), On-target Rate (%), SNP Call Rate (%), Uniformity Rate (%), and Genotype Concordance Rate (%)
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