GenoBaits™
Liquid Probe Capture and Sequencing
Our GBTS GenoBaits technology utilizes liquid probe capture based on probe hybridization and sequence capture. It is suitable for densities ranging from 100 to 200K and has no minimum sample requirement. This method is effective for complex genomes and can detect SNPs, indels, CNVs, and SVs. Additionally, custom panel compositions are available to meet your specific research needs.
Want to learn more about our GBTS marker panel? Click here
Curious about how other researchers have used GBTS? Check out peer-reviewed papers.
GenoPlex™
Multiplexing PCR and Sequencing
Our GBTS GenoPlex technology is based on multiplexing PCR and sequencing, designed for target densities ranging from 20 to 5,000. It allows for over 5,000 PCR reactions in a single tube and is compatible with multiple sequencer platforms. This method effectively targets SNPs, exons, and specific genome regions, including SNPs, insertions, and deletions.
Want to learn more about our GBTS marker panel? Click here
Curious about how other researchers have used GBTS? Check out peer-reviewed papers.
The GenoPlex Workflow for 384-Format PCR Setup and SNP Genotyping
The GenoPlex workflow is designed for high-throughput SNP genotyping using a streamlined, plate-based PCR process. Samples are processed in parallel in a standardized format, followed by target amplification and sample indexing to generate uniquely barcoded libraries.
The pooled libraries are then sequenced on an Illumina platform, enabling accurate and consistent genotype calling across all target SNP sites.
GenoPlex-One™
One-Step Multiplexing PCR and Sequencing
GenoPlex-One is an innovative genotyping solution designed to streamline the multiplexing of thousands of DNA samples while analyzing hundreds of SNPs in a single PCR step. This approach allows for pooling in one reaction for NGS sequencing.
Focusing on efficiency, high-throughput capabilities, and accuracy, GenoPlex-One enables researchers to conduct large-scale genetic testing - all within a single day.
Want to learn more about our GBTS marker panel? Click here
Curious about how other researchers have used GBTS? Check out peer-reviewed papers.
Key Features
- One-Step Multiplexing: Genotype dozens to hundreds of SNPs in a single PCR reaction.
- Flexible Target Design: Cost-effective targeted panels supporting 20–500 markers.
- Simplified, Cost-Efficient Workflow: One PCR stage, one plate—no transfers, lower contamination risk and costs.
- Ultimate Throughput: Process 6,000–10,000 samples per day for large-scale genotyping.
- Integrated Data Analysis: End-to-end SNP analysis powered by MolBreeding.
Following a one-step PCR amplification (~2.5 hours) and a quick pooling/purification step (~1 hour), this process allows for the genotyping of
hundreds of SNPs in a single, efficient workflow.
Performance
In the evaluation of three GenoPlex-One panels—Maize, Pepper, and Tomato—demonstrated consistently high performance, with call rates and uniformity exceeding 94%. This performance was reliably maintained across replicates. All panels achieved over 90% alignment to the reference genome with comparable call rates.
The Performance of three Genoplex-One panels:
Bars are colored from light to dark (left → right) to indicate : Alignment Rate (%), On-target Rate (%), SNP Call Rate (%), Uniformity Rate (%), and Genotype Concordance Rate (%)
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