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Frequently Asked Questions
To help you find the information you need quickly and easily, we've curated answers to some of the most frequently asked questions. We hope this page provides the clarity you're looking for. If you can't find what you're looking for, please don't hesitate to contact us — our team will be happy to assist you.
Sample Preparation
- What kind of samples can be submitted for DNA extraction and library construction?
Accepted plant samples include freeze-dried leaves, seeds, seedlings, and root tissues. Livestock samples can include blood, ear or tail tissues (pigs), muscle tissue, or hair follicles. Aquatic animal samples can be muscle tissue or shrimp antennae.
- How should samples be prepared and shipped to MolBreeding Labs?
Detailed step-to-step guidelines are provided in the sample preparation guides:
Sample preparation and shipping guideline for Leaf Sample
Sample preparation and shipping guideline for Animal Tissue
A completed sample information sheet (Tubes or Plates) should accompany each submission.
- Can genomic DNA be directly submitted for GBTS? What is the recommended DNA concentration and purity for submission?
Yes, the extracted DNA can be submitted directly to MolBreeding, please note that
For reliable genotyping with ready-to-use panels, DNA concentration should be above 5 ng/µL in a 50 µL volume. Lower concentrations often lead to poor library quality, reduced call rates (<70%), and compromised data quality.
- Is there a minimum number of samples required for genotyping in a single batch?
Although MolBreeding does not impose a strict minimum batch size, submitting at least 10 samples is recommended. For whole-genome sequencing, batch size can be discussed based on project requirements.
- Are services available for large-scale breeding programs?
Yes, MolBreeding workflows are scalable to accommodate both small research projects and large breeding programs involving thousands of samples. MolBreeding’s automated laboratory system can handle over 8,000 samples daily.
Panels & Customization
- What kind of variants can be detected using GBTS?
Ready-to-use panels typically detect SNPs and small InDels. Customized panels can also target larger InDels and structural variants.
- How is pricing determined?
Pricing for panels depends on the marker density and the number of samples. For a detailed quote, please contact us at cs@molbreeding.com.
- Is it possible to design a custom SNP panel?
Yes. Custom panels can be developed for any species, either from a provided variant list or by adding desired variants to one of our ready-to-use panels.
- Can panels be updated or expanded after initial design?
MolBreeding GBTS panels are modular and flexible, allowing targets to be added or removed to meet new requirements anytime.
- What are core SNPs and mSNPs in the GBTS？
In GBTS, core SNPs are the primary target markers selected for probe design and are essential for accurate and consistent genotyping. mSNPs (multi-SNPs) arise when multiple SNPs are clustered within the same probe-binding region, allowing additional variants to be captured alongside the core SNP.
- Are there trait-related markers included in the panels?
Some of MolBreeding’s panels include trait-related markers., please inquire cs@molbreeding.com about desirable panels.
- Are marker locations included in the panel available?
Yes, information on marker positions and related details in the panel can be provided upon request.
- Can a different reference genome be used for genotyping?
Yes, the reference genome can be updated based on the specific genome provided for the project.
Processing
- Is it possible to run pilot samples?
Yes. A small pilot batch can be arranged; please discuss the details with the project manager.
- Are repeat submissions or re-sequencing possible in case of failed samples?
Yes. Failed samples are automatically retested once before final reporting. Samples that still fail after the repeat will not be retested further.
- What quality control measures are applied during processing?
All samples undergo strict QC steps, including DNA integrity assessment, library QC, and genotyping accuracy verification. For DNA with low concentration, one purification would be performed to improve it.
Data Delivery & Turnaround Time
- What is the general turnaround time for results?
Turnaround depends on the service and batch size. For general project, results are typically available within 2–3 weeks after sample submission.
- How is analysis data delivered？
Once the project start, a private customer account will be generated on MolBreeding’s Customer Portal. All analysis data is delivered via SFTP and available through the Customer Portal for secure and easy access.
- In which file formats are results provided?
Raw sequencing data, GVCF files for all samples and a summary report are provided for each project. Other equivalent files (e.g., VCFs or genotyping matrices) can also be provided upon request.
- Are advance analysis services available?
Yes. Advance bioinformatic analysis services, including genotype imputation, GWAS, homogeneity analysis for inbreeding values, population diversity analysis, genome selecton, parental identification, functional site analysis, etc., are available to support downstream analysis.
- Is data confidentiality maintained?
All samples and data are treated as strictly confidential, securely stored, and shared only with authorized personnel.
- How long is the data stored?
Raw sequencing data, GVCFs and genotype (GT) files are stored permanently on MolBreeding servers. Other analysis data is stored for 45 days after delivery.
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