Gene-Editing PCTSeq Service
Our Probe-Captured Targeted Sequencing (PCTS) service offers high-accuracy sequencing of gene edits, from single-base modifications to large knock-ins, optimized for cost-effective large-scale sample analysis.
Introduction
The emergence of gene editing technologies, especially CRISPR/Cas9, has revolutionized genetic research and therapeutic applications. However, accurately assessing the outcomes of these edits, particularly in multi-site editing scenarios, presents significant challenges. Our Gene Editing Site Sequencing Service employs a PCTS approach to provide a comprehensive view of edited sequences, capturing variations across hundreds or thousands of samples, thereby enhancing the understanding of gene editing results.
Q: How does the PCTSeq work?
Our proposed method, PCTS, leverages the following key components:
1. Custom Probe Design: Specific probes are designed to target the regions of interest, ensuring high specificity for the edited sites while accommodating sequence diversity.
2. Hybridization and Capture: The probes hybridize to the target sequences, allowing for efficient capture of the edited regions.
3. Next-Generation Sequencing (NGS): The captured sequences are sequenced using NGS technology, providing a comprehensive view of all edited sites across a large number of samples.
Key Benefits:
- Comprehensive Coverage: PCTS captures all edited sites, providing a full view of the genetic modifications made.
- High Sensitivity: The method is highly sensitive, allowing for the detection of low-frequency variants, and different versions of edited sites.
- Scalability: PCTS is designed to handle hundreds or thousands of samples simultaneously, making it suitable for large-scale projects.
- Cost-Effectiveness: By streamlining the sequencing process, PCTS reduces costs associated with data generation and analysis.
Customer Testimonials: "We are pleased with our first trial run using Molbreeding’s high-throughput genotyping service, particularly regarding the data quality and the service your team provided. This approach has saved us considerable time and effort in our multi-gene editing projects, while offering significant budget savings compared to conventional sequencing methods and maintaining accuracy or reliability."
Our Service Workflow
Our PCTS Service workflow is streamlined to deliver fast, accurate results:
1. Sample Preparation: DNA is extracted from the samples, and libraries are prepared for sequencing.
2. Probe Hybridization: Custom probes are introduced to hybridize with the target gene editing sites.
3. Sequence Capture: Hybridized sequences are captured and purified for sequencing.
4. Sequencing: The captured DNA is sequenced using NGS platforms, generating detailed sequence data.
5. Data Analysis: Advanced bioinformatics tools are utilized to analyze the sequence data, identifying edited sites, variations, and off-target effects.
Report Visualization
The detection report displays the haplotype distribution of target regions across various samples, providing a clear interpretation of mutation frequency and sequencing depth in the gene editing areas.
Detection of Various Mutation Types
Large Deletion Results in Target Regions
Applications
Our Gene Editing Site Sequencing Service is optimized for large-scale sample analysis, making it an ideal solution for high-throughput genetic research. Key applications include:
- Agricultural Biotechnology: Evaluate genetic modifications in crops to enhance traits like yield, disease resistance, and environmental adaptability.
- Therapeutics: Evaluating the efficacy and safety of gene editing cell lines in clinical settings.
- Basic Research: Explore gene function and regulation with precise sequence analysis.
The future of gene editing with PCTSeq
The PCTS-powered Gene Editing Site Sequencing Service is transforming the landscape of genetic research. By delivering detailed insights into edited sequences across multiple sites and thousands of samples, we’re enabling researchers to achieve more precise and impactful outcomes. From enhancing crop resilience to advancing precision medicine, our service is driving innovation and unlocking new possibilities in gene editing.
Get Started Today！
We invite researchers, institutions, and biotech companies to collaborate with us in utilizing this innovative sequencing approach to enhance their gene editing projects. For more information or to schedule a consultation, please contact us.
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